Fluorimetric Method for the Determination of Erythromycin using a Photochemical Derivatization Approach

نویسندگان

  • Virginia de L. M. Finete
  • Márcia Arissawa
  • Ricardo Q. Aucélio
چکیده

A derivação fotoquímica é proposta como abordagem para induzir fluorescência intensa (412/465 nm) da eritromicina. Parâmetros experimentais importantes como o tempo de irradiação com UV e tipo e concentração de ácido usado para tratar a solução de analito foram ajustadas. Limites de detecção e quantificação de 0,025e 0,085 μg mL foram obtidos com resposta linear até 200 μg mL. O procedimento é seletivo em relação a antibióticos aminoglicosídicos (canamicina, gentamicina e amicacina). O método foi testado em formulações farmacêuticas e em uma vacina contendo eritromicina como conservante, com recuperações do analito entre 98 e 105%.

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تاریخ انتشار 2008